Myocardial infarction was induced in rats by subcutaneous injection of isoproterenol 200 mgkg for two consecutive days at an interval of 24 h. As it has been reported that isoproterenol can cause ischemic myocardial alterations, lipid peroxide generation and procoagulant activity, we administered it to rabbits in order to induce a noninvasive myocardial infarction associated with above mentioned. Isoproterenolinduced myocardial infarction in rats. Pdf myocardial damage induced by a single high dose of. A biochemical, electrocardiographic and histoarchitectural evaluation. Tomato lycopene attenuates myocardial infarction induced. In the present study, we investigated the effect of dg postconditioning on isoproterenol iso induced myocardial injury in rats.
Evaluation of cardioprotective effect of tinospora. Original article ginsenoside rg2 attenuated myocardial injury induced by isoproterenol in rats zhigang ma 1, xiaoli fan, gaowa eerdun3, pinyuan zhang2 1department of combine traditional chinese and western medicine, yantai yuhuangding hospital affiliated to qin. High carbohydrate diets enhances lipogenesis and reduces. Subcutaneous injection of iso 85 mgkg body weight b. Myocardial and serum lactate changes during isoproterenol induced infarction robert b. Cardioprotective effects of puerarinv on isoproterenol. Use of isoproterenol hydrochloride injection is contraindicated in patients with tachyarrhythmias. Abstract mature male rats with isoproterenol induced myocardial infarction. Myocardial infarction was induced by isoproterenol in adult male rats, as a single intraperitoned injection at a dose of 150mg kg body weight the induced myocardial infarction. Cincinnati, ohio severe myocardial infarction can be induced in rats by two subcutaneous injections of the potent betaadrenergic stimulating agent, isoproterenol.
Myocardial damage induced by a single high dose of isoproterenol in c57bl6j mice triggers a persistent adaptive immune response against the heart. It has copious health benefits such as improving digestion, stimulating metabolism, and exhibits antioxidant and antiinflammatory effects. Salacia chinensis plant extract evaluated the effect of isoproterenol induced myocardial infarction in rats. Iso was injected intraperitoneally 200 mgkg to induce acute 26 hours myocardial injury in. This study was designed to examine its cholesterol lowering, antithrombotic and antiinflammatory effects. Our study is aimed at evaluating the effects of pretreatment with polylactic. Protective effect of vitis vinifera against myocardial.
Cardioprotective effect of melatonin against isoproterenol induced myocardial infarction in rats. In the present study, we evaluated the role of ritonavir in isoproterenol induced. Original article ginsenoside rg2 attenuated myocardial injury. The main cause of death from chd is myocardial infarction mi, which is an ischemic necrosis of cardiac myocytes results from prolonged interruption of blood supply to certain area of heart. Eplerenone attenuates myocardial infarction in diabetic. Modulation of serum adropin levels by isoproterenol induced acute myocardial infarction in normal and obese male albino rats. Original article baicalin ameliorates isoproterenol induced acute myocardial infarction through inos, inflammation, oxidative stress and p38mapk pathway in rat shenjie sun, xiaopeng wu, hengliang song, guiqi li department of cardiology, the second hospital affiliated to zhengzhou university, zhengzhou 450014, henan, china. Isoproterenol definition and meaning collins english dictionary.
To achieve the most comprehensive screening of the entire metabolome related to isoproterenol iso induced mi, we present a tissue targeted metabonomic study using an integ. To test the effect of a tricolor on isoproterenol induced. Genetic dissection of cardiac remodeling in an isoproterenol. Moreover, we show that upregulation of antioxidant enzymes, most of which are regulated by the enhanced keap1nrf2 signaling. When sildenafil was injected daily, one hour before isoproterenol, survival was significantly improved and the myocardium didnt show significant hypertrophy or cell injury. Role of nitric oxide in isoproterenolinduced myocardial. Amelioration of inflammation by phenolic rich methanolic. Myocardial infarction induced by isoproterenol iso. Department of biochemistry, university of madras, guindy campus chennai 600 025, india received 24 june 2005. Conversely, daily subcutaneous administration of isoproterenol for 10 days caused significant myocardial hypertrophy, cell injury and decline in survival. Myocardial infarction was induced by subcutaneous injection of isoproterenol, 85 mgkg body weight after a pretreatment period of 45 days with eriodictyol in various doses 50 mg, 100 mg and 200 mg per kg body weight through intragastric intubation. The acute phase of myocardial necrosis induced by isoproterenol mimics changes in blood pressure, heart rate, electrocardiogram ecg, and left ventricular dysfunction similar to that occurring in patients with myocardial infarction.
Vinpocetine is a phosphodiesterase type1 inhibitor having strong vasodilating effect through camp and cgmp elevation. Cardioprotective potential of myricetin in isoproterenol. Vasodilators may play positive role in myocardial infarction mi. Curcumin nanoparticles protect against isoproterenol. However, the effect of ritonavir on cardiac glut4 inhibition during myocardial necrosis is not investigated. Iso isoproterenol each group contains 5 animals either sex induction of myocardial infarction myocardial infarction was induced by isoproterenol hydrochloride 85mgkg body weight, dissolved in normal saline given through subcutaneous injection for two consecutive days 29th and 30th. The present study evaluated cardioprotective effect of lyophilized hydroalcoholic extract of moringa oleifera in the isoproterenol isp induced model of myocardial infarction. Experimental model of myocardial infarction induced by.
Pdf origination and development of isoproterenolinduced. Adult male wistar albino rats were pretreated with sundarban honey 5 gkg daily for a period of 6 weeks. Wistar male albino rats were randomly divided and treated with extract of cardamom 100 and 200 mgkg per oral or normal saline for 30 days with. Myocardial infarction is usually induced in small animals by means of invasive techniques based on mechanical coronary obstruction. Rice, cassava and corn starch are some of the importanthcds used worldwide. The effect of astragaloside iv on isoproterenol induced myocardial infarction in rats article pdf available in pharmaceutical sciences 252.
Original article baicalin ameliorates isoproterenolinduced. Ginsenoside rg3 protects heart against isoproterenol induced myocardial infarction by activating ampk mediated autophagy guizhi sun, fanji meng, huaiqiu cai, xuebo diao, bo zhang, xiuping bai. Myocardial and serum lactate changes during isoproterenol. Preventive effect of naringin on cardiac markers, electrocardiographic patterns and lysosomal hydrolases in normal and isoproterenolinduced. Amelioration of isoproterenolinduced oxidative damage in rat.
Myocardial infarction mi is a leading cause of morbidity and mortality but the precise mechanism of its pathogenesis remains obscure. We performed microarray analyses on rna from mice with isoproterenol. Research article amelioration of isoproterenolinduced. The study was conducted to evaluate the cardioprotective activity of combination comb of syringic acid sa and resveratrol rv against isoproterenol iso induced cardiotoxicity in rats. Dailymed isoproterenol hydrochloride injection, solution. Ibrahimkhalil, 1,2 istiyakahmmed, 1 romanaahmed, 1 e. Zorn, fosberg moraceae are used in the management of hypertension. Isoproterenol lnduced myocardial infarction in rats distribution of corticosterone by jack saroff, ph. Amelioration of inflammation by phenolic rich methanolic extract of ocimum sanctum linn. The localization of tritiated isp in the damaged myocardium of rats has been recently described.
Experimental myocardial infarction was induced by injecting isoproterenol iso hydrochloride dissolved in physiological solution subcutaneously to rats. Marwa a o, fatma eln, a elsafty, wael b elkhol, yabeer e elm. The isoproterenol induced myocardial infarction in rodents offers a relevant model to study the effect of natural products. On the days th and 14 th of study protocol, isoproterenol iso 100 mgkg was injected subcutaneously to rats with 24 h of interval to induce. Protection effect of wenxin keli on isoproterenol induced. Isoproterenolinduced myocardial infarction in rabbits. Since h3isp was noted in grooves along the sarcolemma of ischemic and necrotic fibers, an action on the membrane with exaggerated calcium inflow. Isoproterenol iso induced mi is a wellestablished animal model of pharmacologically induced mi. We here describe the shortterm effects in rabbit of isoproterenol on the parameters associated with myocardial necrosis histology, ecg, plasma cardiac necrosis markers, or involved in peroxidation.
A single subcutaneous injection of isoproterenol 85 mgkg results in a rapid, marked increase in infarctlike myocardial necrosis in rats. The study aimed to evaluate the protective role of myricetin obtained from vitis vinifera vitaceae on heart rate, electrocardiographic ecg patterns, vascular reactivity to catecholamines, cardiac marker enzymes, antioxidant enzymes together with morphological and histopathological changes in isoproterenol iso induced myocardial infarction. We investigated the protective role of withania somnifera leaf extract wslet on isoproterenol iso induced myocardial infarction mi in rats. Combined cardioprotective ability of syringic acid and. The specific marker enzymes for myocardial infarction were studied in the serum samples to determine the degree of infarction. Acute treatment with metformin improves cardiac function. Pdf the effect of astragaloside iv on isoproterenol. Moringa oleifera leaf extract prevents isoproterenol. Ginsenoside rg3 protects heart against isoproterenolinduced. Induction of myocardial infarction the myocardial infarction was induced in experimental rats by intraperitoneal i. Effect of exercise and dietary polyunsaturated fat upon.
Cardioprotective effect of amaranthus tricolor extract in. Myocardial lesions induced by isoproterenol isp are similar to those of human coagulative myocytolysis or myofibrillar degeneration. Effects of heme oxygenase1 upregulation on isoproterenol. Enhanced keap1nrf2 signaling protects the myocardium from. It was hypothesized that endurance exercise training would attenuate isoproterenolinduced myocardial necrosis in the rat by increasing the concentration of prostacyclin in the myocardial vasculature. Iso isoproterenol each group contains 5 animals either sex induction of myocardial infarction myocardial infarction was induced by isoproterenol hydrochloride 85mgkg body weight, dissolved in. Preventive effect of agnucastoside c against isoproterenol induced myocardial injury article pdf available in scientific reports 71 december 2017 with 202 reads how we measure reads. Sixty sixweek old male wistar rats were randomized into six groups. In general, mi occurs unexpectedly and the clinical. Using a panel of diverse inbred mouse strains, treated with a. Original article baicalin ameliorates isoproterenol. Experimental protocol the rats were randomly divided onto three groups with six rats each. Cardioprotective effects of lagenaria siceraria fruit juice. Therefore, mi in animal models studies should be induced in conscious animals with intact reflexes for greater clinical re levance.
Pdf isoproterenolinduced myocardial infarction in wistar rats. Isoproterenolinduced 45ca uptake into myocardium of rats. The main mechanism of isoproter enolinduced myocardial ischemia is the gen eration of free radicals and reactive oxygen species, lipid peroxidation, oxidative stress, calcium overload, etc 7. Isoprenaline, or isoproterenol, is a medication used for the treatment of bradycardia slow heart rate, heart block, and rarely for asthma. Baicalin and isoproterenolinduced acute myocardial infarction. Abstractthe present study was designed to evaluate the cardioprotective effects of lagenaria. Reports showed that ispinduced myocardial metabolic alterations are similar to those occurring in human beings following a myocardial infarction19,20. Rice is grown worldwide and provides food for more than half of. Based on the results presented, we conclude that the model of myocardial infarction induced by isoproterenol in rats was adequately reproduced and validated in our laboratory and caused increased mortality and myocardial injury markers ast and troponin i, reduction of catalase activity and levels of glutathione and histopathological changes. The rat model of isoproterenol induced myocardial infarction offers. Cardioprotective effect of ritonavir, an antiviral drug, in. Isoproterenol group ii induced the oxidative myocardial damage via alteration in the endogenous antioxidant enzymes and myocardial marker enzymes.
Sildenafil citrate increases myocardial cgmp content in rat. Mature male rats with isoproterenol induced myocardial infarction and normal controls were injected intravenously with 1, 23 h corticosterone 3 h corticosterone. This model becomes relevant if we consider that increased adrenergic. To study the treatment effect of wenxin keli on isoproterenol iso induced heart failure in rats. This study was designed to scientifically evaluate the effects of an aqueous extract of muntingia calabura l. Original article evening primrose oil ameliorates platelet. Cardioprotective effect of tincture of crataegus on.
He lin, zifeng pi, lihui men, weijia chen, zhiqiang liu, zhongying liu. Isoproterenolinduced myocardial injury and diastolic. We proposed that evening prime rose oil epo can improve the outcome of a heart with myocardial infarction mi in the presence of diet induced hyperaggregability. Myocardial infarction induced by isoproterenol iso using toxic concentration of this. Possible effect of natural honey on isoproterenol induced. Exercise training, indomethacin, and isoproterenolinduced.
Rats were randomly assigned to exercise and control groups. Pdf evaluation of cardioprotective role of vinpocetine. Studies on the beneficial effects of ethanolic extract and. Isoprenaline isoproterenol is a synthetic catecholamine and. Isoprenalineinduced changes in regional myocardial. High fat diet was administered for 4 weeks then mi was induced by. Metabonomic study of chinese medicine shuanglong formula as. Isoproterenol group ii induced the oxidative myocardial damage via alteration in the endogenous antioxidant enzymes and myocardial.
The current study investigated the effect of cardamom on hemodynamic, biochemical, histopathological and ultrastructural changes in isoproterenol iso induced myocardial infarction. In this study, we address this issue using a novel transgenic mouse model for nrf2 regulation in the context of an isoproterenol induced myocardial remodeling. In addition to its antiviral effect, ritonavir directly inhibits the insulinregulated glucose transporter glut4 and blocks glucose entry into fat and muscle cells. Pdf cardioprotective potential of ocimum sanctum in. Myocardial infarction, antioxidants, isoproterenol, natural products. Interstitial fibrosis significantly was attenuated in treated groups compared with control mi group. Danshengegen decoction dg, a chinese herbal formula, has been demonstrated to be effective for the treatment of coronary heart disease such as myocardial infarction. The metabolic disturbances of isoproterenol induced. The standard drug metoprolol succinate was administered orally at a dose of 2. The present study was designed to investigate the cardioprotective effects of sundarban honey sh in rats with isoproterenol iso induced myocardial infarction. This defect in perfusion persists for up to 150 min and corresponds in distribution to the myocardial necrosis produced by similar doses of isoprenaline. Other words that entered english at around the same time include. Effect of cardamom on histopathology in the isoproterenolinduced myocardial infarction in rats.
The main mechanism of isoproter enolinduced myocardial ischemia. Bioflavonoid hesperidin possesses the antihyperglycemic. In this study, we compared the transcriptional response of the heart in this model to other animal models of heart failure, as well as to the transcriptional response of human hearts suffering heart failure. These hunches have accelerated the need for natural products, which may be used as dietary supplement to prevent the development of an acute myocardial infarction. It is mediated by the betareceptors as it is prevented by betablockade with propranolol. Protective effects of cardamom in isoproterenolinduced. Myocardial infarction mi is an important cause of mortality around the world, resulting from an ischemic necrosis induced by a vascular occlusion. Research article amelioration of isoproterenol induced oxidative damage in rat myocardium by withania somnifera leaf extract md. Origination and development of isoproterenolinduced myocardial infarction in male wistar rats article pdf available in international research journal of pharmacy 45. A normal group received saline showing normalarchitecture of myocardium. We had previously reported isoproterenol induced cardiac phenotypes in the hmdp.
Previous largescale human genomewide association studies have yielded only a handful of genetic loci contributing to heart failurerelated traits. Cardioprotective effect of mangiferin on isoproterenol. Author summary heart failure is the most common cause of morbidity and mortality in the aging population. Ginkgo biloba extract in all three dose group iii, iv and. It is considered that isoprenalineinduced myocardial necrosis represents myocardial infarction. Declaration i hereby declare that this thesis entitled studies on the beneficial effects of ethanolic extract and total alkaloids from whole plant of boerhaavia diffusa on isoproterenol induced myocardial infarction. Possible effect of natural honey on isoproterenol induced acute myocardial infarction. Isoproterenol induced cardiac hypertrophy in mice has been used in a number of studies to model human cardiac disease. The present study was done to investigate the protective effect of tcr on experimentally induced myocardial infarction in rats. Evaluation of cardioprotective effect of tinospora cordifolia. The e ect of pretreatment with curcumin nanoparticles nc compared to conventional curcumin cs on blood pressure, electrocardiogram, and biological changes on isoproterenol iso induced myocardial infarction.
536 1475 504 954 1134 1358 163 1378 1546 722 686 1076 566 342 1390 1234 281 367 158 27 988 780 1457 767 495 629 56 1160 824 55